Recombinant human HES2 protein

Hairy and enhancer of split 2, also known as HES2, is a member of the HES family and contains one basic helix-loop-helix (bHLH) domain and one orange domain. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. Activation of Notch signaling pathway leads to activation of HES family genes through the interaction between Notch intracellular domain and RBPSuH (CSL) . HES2 is expressed in a variety of adult and embryonic tissue. Recombinant human HES2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Transcription factor HES-2, bHLHb40, hairy and enhancer of split 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

20.9 kDa (196aa)

Additionnal Information

HES2, Transcription factor HES-2, bHLHb40, hairy and enhancer of split 2, ATGP2260-10 µg, ATGP2260-20 µg, ATGP2260-50 µg, ATGP2260-100 µg, ATGP2260-250 µg, ATGP2260-500 µg, ATGP2260-1 mg, ATGP2260-10, ATGP2260-20, ATGP2260-50, ATGP2260-100, ATGP2260-250, ATGP2260-500, ATGP2260-1

References & Citations

Akazawa C., et al. (1992) J Biol Chem. 267:21879-21885.; ; Ishibashi M., et al. (1993) J Biochem. 215:645-652.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_061962

Uniprot Accession Number

Q9Y543

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW