Recombinant human mesotrypsin/PRSS3 protein

PRSS3 is a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. Recombinant human PRSS3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Brain trypsinogen, Mesotrypsin, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin-3, Trypsin III, Trypsin IV, PRSS4, TRY3, TRY4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26 kDa (247aa)

Additionnal Information

PRSS3, Trypsin-3 isoform 1, MTG, PRSS4, T9, TRY3, TRY4, ATGP2255-10 µg, ATGP2255-20 µg, ATGP2255-50 µg, ATGP2255-100 µg, ATGP2255-250 µg, ATGP2255-500 µg, ATGP2255-1 mg, ATGP2255-10, ATGP2255-20, ATGP2255-50, ATGP2255-100, ATGP2255-250, ATGP2255-500, ATGP2255-1

References & Citations

Masson E, Le Marechal C, et al. (2008) . Pancreas. 37 (2) :227-8. ; ; Jiang G, Cao F, et al. (2010) . Gut. 59 (11) :1535-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_031369.2

Uniprot Accession Number

P35030

Species

Human

AA Sequence

IVGGYTC EENSLPYQVS LNSGSHFCGG SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN RDTLDNDIML IKLSSPAVIN ARVSTISLPT APPAAGTECL ISGWGNTLSF GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANS