Recombinant human EMP/MAEA protein

Macrophage erythroblast attacher isoform 1, also known as MAEA, is a 396 amino acid ubiquitously expressed adhesion protein. Expressed as 5 alternatively spliced isoforms, MAEA contains one CTLH domain and one LisH domain. MAEA can form a complex with F-actin, which is involved regulating actin distribution in erythroblasts and macrophages. Considered to assist with cell division and nuclear architecture, MAEA is localized with condensed chromatin at prophase, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. Recombinant human MAEA protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Macrophage erythroblast attacher isoform 1, EMLP, EMP, GID9, HLC-10, PIG5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

47.7 kDa (419aa)

Additionnal Information

EMP, MAEA, Macrophage erythroblast attacher isoform 1, EMLP, EMP, GID9, HLC-10, PIG5, ATGP2251-10 µg, ATGP2251-20 µg, ATGP2251-50 µg, ATGP2251-100 µg, ATGP2251-250 µg, ATGP2251-500 µg, ATGP2251-1 mg, ATGP2251-10, ATGP2251-20, ATGP2251-50, ATGP2251-100, ATGP2251-250, ATGP2251-500, ATGP2251-1

References & Citations

Soni S., et al. (2006) J Biol Chem. 281:20181-20189.; ; Bala S., et al. (2006) Biochem Biophys Res Commun. 342:1040-1048.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001017405

Uniprot Accession Number

Q7L5Y9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAVQESA AQLSMTLKVQ EYPTLKVPYE TLNKRFRAAQ KNIDRETSHV TMVVAELEKT LSGCPAVDSV VSLLDGVVEK LSVLKRKAVE SIQAEDESAK LCKRRIEHLK EHSSDQPAAA SVWKRKRMDR MMVEHLLRCG YYNTAVKLAR QSGIEDLVNI EMFLTAKEVE ESLERRETAT CLAWCHDNKS RLRKMKSCLE FSLRIQEFIE LIRQNKRLDA VRHARKHFSQ AEGSQLDEVR QAMGMLAFPP DTHISPYKDL LDPARWRMLI QQFRYDNYRL HQLGNNSVFT LTLQAGLSAI KTPQCYKEDG SSKSPDCPVC SRSLNKLAQP LPMAHCANSR LVCKISGDVM NENNPPMMLP NGYVYGYNSL LSIRQDDKVV CPRTKEVFHF SQAEKVYIM