Recombinant human ATP5H protein
ATP synthase subunit d, also known as ATP5H, is a 161 amino acid protein that belongs to the ATPase d subunit family. ATP5H encodes the d subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane, ATP5H exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17q25. 1. Recombinant human ATP5H protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
ATP synthase subunit d mitochondrial isoform a, ATP synthase subunit d, mitochondrial isoform a, ATPQ
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
20.9 kDa (184aa)
Additionnal Information
References & Citations
Noh H S., et al. (2004) Brain Res. 129:80-87.; ; Sansanwal P., et al. (2010) J Am Soc Nephrol. 21:272-283.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_006347
Uniprot Accession Number
O75947
Species
Human
AA Sequence
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