Recombinant human Trappin-2/Elafin protein
PI3 is an elastase-specific protease inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. PI3 belongs to the centromeric cluster. Recombinant human PI3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Elafin preproprotein, Cementoin, ESI, SKALP, WAP3, WFDC14
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
12 kDa (118aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sumi Y, Inoue N, et al. (2002) . Atherosclerosis. 160 (1) :31-9.; ; Kuijpers AL, Pfundt R, et al. (1998) . Clin Genet. 54 (1) :96-101.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_002629
Uniprot Accession Number
P19957
Species
Human
AA Sequence
AVTGVPV KGQDTVKGRV PFNGQDPVKG QVSVKGQDKV KAQEPVKGPV STKPGSCPII LIRCAMLNPP NRCLKDTDCP GIKKCCEGSC GMACFVPQ
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