Recombinant human USP15 protein

USP15 is a member of the ubiquitin specific protease (uSP) family of deubiquitinating enzymes. uSP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. Recombinant human uSP15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 15 isoform 3, uNPH-2, uNPH4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.5 kDa (258aa) confirmed by MALDI-TOF

Additionnal Information

USP15, ubiquitin carboxyl-terminal hydrolase 15 isoform 3, uNPH-2, uNPH4, ATGP2237-10 µg, ATGP2237-20 µg, ATGP2237-50 µg, ATGP2237-100 µg, ATGP2237-250 µg, ATGP2237-500 µg, ATGP2237-1 mg, ATGP2237-10, ATGP2237-20, ATGP2237-50, ATGP2237-100, ATGP2237-250, ATGP2237-500, ATGP2237-1

References & Citations

Imboden, M., et al. (2012) J. Allergy Clin. Immunol. 129 (5), 1218-1228; ; Eichhorn, P.J., et al. (2012) Nat. Med. 18 (3), 429-435

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001239008

Uniprot Accession Number

Q9Y4E8

Species

Human

AA Sequence

MAEGGAA DLDTQRSDIA TLLKTSLRKG DTWYLVDSRW FKQWKKYVGF DSWDKYQMGD QNVYPGPIDN SGLLKDGDAQ SLKEHLIDEL DYILLPTEGW NKLVSWYTLM EGQEPIARKV VEQGMFVKHC KVEVYLTELK LCENGNMNNV VTRRFSKADT IDTIEKEIRK IFSIPDEKET RLWNKYMSNT FEPLNKPDST IQDAGLYQGQ VLVIEQKNED GTWPRGPSTP KKPLEQSC