Recombinant human IL-36 gamma/IL-1F9 protein

IL36G is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta) . Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Recombinant human IL36B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-36 gamma, IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1, IL1RP2, Macrophagleukin-36 gamma, 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.1 kDa (192aa) confirmed by MALDI-TOF

Additionnal Information

IL36 g, IL1F9, Interleukin-36 gamma, IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1, IL1RP2, ATGP2236-10 µg, ATGP2236-20 µg, ATGP2236-50 µg, ATGP2236-100 µg, ATGP2236-250 µg, ATGP2236-500 µg, ATGP2236-1 mg, ATGP2236-10, ATGP2236-20, ATGP2236-50, ATGP2236-100, ATGP2236-250, ATGP2236-500, ATGP2236-1

References & Citations

Kumar S., et al. (2000) J. Biol. Chem. 275:10308-10314; ; Debets R., et al. (2001) J. Immunol. 167:1440-1446

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_062564

Uniprot Accession Number

Q9NZH8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMRGTPGD ADGGGRAVYQ SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND