Recombinant human NCK2 protein
NCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptiona splice variants, encoding different isoforms, have been characterized. Recombinant human NCK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NCK adaptor protein 2, GRB4, NCKbeta
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
45.3 kDa (403aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Braverman L.E., et al. (1999) J. Biol. Chem. 274:5542-5549; ; Latreille M., et al. (2006) J. Biol. Chem. 281:26633-26644
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_003572
Uniprot Accession Number
O43639
Species
Human
AA Sequence
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