Recombinant human HBQ1 protein
Hemoglobin subunit theta-1, also known as HBQ1, belongs to the Hemoglobin family. Hemoglobin (Hgb) is a 66. 7 kDa protein coupled to four iron-binding, methenelinked tetrapyrrole rings (heme) . The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane. Recombinant human HBQ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Hemoglobin subunit theta-1, hemoglobin, theta 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.9 kDa (165aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liebhaber S A., et al. (1981) Nature. 290:26-29; ; Sudha R., et al. (2004) J biol Chem. 279:20018-20027.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Cardiovascular
NCBI Accession Number
NP_005322
Uniprot Accession Number
P09105
Species
Human
AA Sequence
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