Recombinant human HLA-C protein

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. Recombinant human HLA-C protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HLA class I histocompatibility antigen Cw-1 alpha chain, HLA class I histocompatibility antigen, Cw-1 alpha chain, D6S204, HLA-JY3, HLC-C, PSORS1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.9 kDa (307aa) confirmed by MALDI-TOF

Additionnal Information

HLA-C, ATGP2222-10 µg, ATGP2222-20 µg, ATGP2222-50 µg, ATGP2222-100 µg, ATGP2222-250 µg, ATGP2222-500 µg, ATGP2222-1 mg, ATGP2222-10, ATGP2222-20, ATGP2222-50, ATGP2222-100, ATGP2222-250, ATGP2222-500, ATGP2222-1

References & Citations

Xu Y., et al. (2009) Tissue Antigens. 74:453-455; ; Zou H.Y., et al. (2009) Tissue Antigens. 74:455-456

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_002108

Uniprot Accession Number

P10321

Species

Human

AA Sequence

CSHSMRY FDTAVSRPGR GEPRFISVGY VDDTQFVRFD SDAASPRGEP RAPWVEQEGP EYWDRETQKY KRQAQADRVS LRNLRGYYNQ SEDGSHTLQR MSGCDLGPDG RLLRGYDQSA YDGKDYIALN EDLRSWTAAD TAAQITQRKL EAARAAEQLR AYLEGTCVEW LRRYLENGKE TLQRAEPPKT HVTHHPLSDH EATLRCWALG FYPAEITLTW QRDGEDQTQD TELVETRPAG DGTFQKWAAV VVPSGQEQRY TCHMQHEGLQ EPLTLSWEPS SQPTIPI

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