Recombinant human RPL11 protein
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL11 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribosomal protein L11, DBA7, GIG34, L11
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.6 kDa (201aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mahata, B., et al. (2012) Oncogene 31 (25), 3060-3071; ; Dai, M.S, et al. (2012) J. Biol. Chem. 287 (21), 17120-17129
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT, 2mM EDTA, 250 mM Imidazole
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_000966
Uniprot Accession Number
P62913
Species
Human
AA Sequence
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