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Recombinant human RPL11 protein

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL11 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosomal protein L11, DBA7, GIG34, L11

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.6 kDa (201aa) confirmed by MALDI-TOF

Additionnal Information

RPL11, Ribosomal protein L11, DBA7, GIG34, L11, ATGP2220-10 µg, ATGP2220-20 µg, ATGP2220-50 µg, ATGP2220-100 µg, ATGP2220-250 µg, ATGP2220-500 µg, ATGP2220-1 mg, ATGP2220-10, ATGP2220-20, ATGP2220-50, ATGP2220-100, ATGP2220-250, ATGP2220-500, ATGP2220-1

References & Citations

Mahata, B., et al. (2012) Oncogene 31 (25), 3060-3071; ; Dai, M.S, et al. (2012) J. Biol. Chem. 287 (21), 17120-17129

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT, 2mM EDTA, 250 mM Imidazole

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000966

Uniprot Accession Number

P62913

Species

Human

AA Sequence

MAQDQGE KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKGL KVREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ QKYDGIILPG K
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