Recombinant human NSL1 protein

NSL1 is a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human NSL1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Kinetochore-associated protein NSL1 homolog, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae), C1orf48, DC8, MIS14

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.6 kDa (304aa)

Additionnal Information

NSL1, Kinetochore-associated protein NSL1 homolog, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae), C1orf48, DC8, MIS14, ATGP2214-10 µg, ATGP2214-20 µg, ATGP2214-50 µg, ATGP2214-100 µg, ATGP2214-250 µg, ATGP2214-500 µg, ATGP2214-1 mg, ATGP2214-10, ATGP2214-20, ATGP2214-50, ATGP2214-100, ATGP2214-250, ATGP2214-500, ATGP2214-1

References & Citations

Obuse, Chikashi; ; Iwasaki Osamu, et al. (Nov. 2004) . Nat. Cell Biol. (England) 6 (11) : 1135-41.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_056286

Uniprot Accession Number

Q96IY1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGSPEL VVLDPPWDKE LAAGTESQAL VSATPREDFR VRCTSKRAVT EMLQLCGRFV QKLGDALPEE IREPALRDAQ WTFESAVQEN ISINGQAWQE ASDNCFMDSD IKVLEDQFDE IIVDIATKRK QYPRKILECV IKTIKAKQEI LKQYHPVVHP LDLKYDPDPA PHMENLKCRG ETVAKEISEA MKSLPALIEQ GEGFSQVLRM QPVIHLQRIH QEVFSSCHRK PDAKPENFIT QIETTPTETA SRKTSDMVLK RKQTKDCPQR KWYPLRPKKI NLDT