Recombinant human GRP78/HSPA5 protein

HSPA5 is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. Recombinant human HSPA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

72.9 kDa (659aa)

Additionnal Information

GRP78, HSPA5, 78 kDa glucose-regulated protein, BIP, GRP78, MIF2, ATGP2212-10 µg, ATGP2212-20 µg, ATGP2212-50 µg, ATGP2212-100 µg, ATGP2212-250 µg, ATGP2212-500 µg, ATGP2212-1 mg, ATGP2212-10, ATGP2212-20, ATGP2212-50, ATGP2212-100, ATGP2212-250, ATGP2212-500, ATGP2212-1

References & Citations

Wang Q, Shu R, et al. (2012) . Int J Oncol. 41 (2) :652-60. ; ; Sokolowska I, Woods AG, et al. (2012) . FEBS J. 279 (14) :2579-94.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005338.1

Uniprot Accession Number

P11021

Species

Human

AA Sequence

EEEDKKE DVGTVVGIDL GTTYSCVGVF KNGRVEIIAN DQGNRITPSY VAFTPEGERL IGDAAKNQLT SNPENTVFDA KRLIGRTWND PSVQQDIKFL PFKVVEKKTK PYIQVDIGGG QTKTFAPEEI SAMVLTKMKE TAEAYLGKKV THAVVTVPAY FNDAQRQATK DAGTIAGLNV MRIINEPTAA AIAYGLDKRE GEKNILVFDL GGGTFDVSLL TIDNGVFEVV ATNGDTHLGG EDFDQRVMEH FIKLYKKKTG KDVRKDNRAV QKLRREVEKA KRALSSQHQA RIEIESFYEG EDFSETLTRA KFEELNMDLF RSTMKPVQKV LEDSDLKKSD IDEIVLVGGS TRIPKIQQLV KEFFNGKEPS RGINPDEAVA YGAAVQAGVL SGDQDTGDLV LLDVCPLTLG IETVGGVMTK LIPRNTVVPT KKSQIFSTAS DNQPTVTIKV YEGERPLTKD NHLLGTFDLT GIPPAPRGVP QIEVTFEIDV NGILRVTAED KGTGNKNKIT ITNDQNRLTP EEIERMVNDA EKFAEEDKKL KERIDTRNEL ESYAYSLKNQ IGDKEKLGGK LSSEDKETME KAVEEKIEWL ESHQDADIED FKAKKKELEE IVQPIISKLY GSAGPPPTGE EDTAEKDEL