Recombinant human BSND protein

BSND is an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this BSND have been associated with Bartter syndrome with sensorineural deafness. Recombinant human BSND protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Barttin, BART, DFNB73

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.7 kDa (290aa) confirmed by MALDI-TOF

Additionnal Information

BSND, Barttin, BART, DFNB73, ATGP2204-10 µg, ATGP2204-20 µg, ATGP2204-50 µg, ATGP2204-100 µg, ATGP2204-250 µg, ATGP2204-500 µg, ATGP2204-1 mg, ATGP2204-10, ATGP2204-20, ATGP2204-50, ATGP2204-100, ATGP2204-250, ATGP2204-500, ATGP2204-1

References & Citations

Park, C.W., et al. (2011) Clin. Nephrol. 75 (SuPPL 1), 69-74; ; Iqbal, H., et al. (2011) J. Biomed. Biotechnol. 2011, 304612;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_476517

Uniprot Accession Number

Q8WZ55

Species

Human

AA Sequence

CQCYPKI TFVPADSDFQ GILSPKAMGL LENGLAAEMK SPSPQPPYVR LWEEAAYDQS LPDFSHIQMK VMSYSEDHRS LLAPEMGQPK LGTSDGGEGG PGDVQAWMEA AVVIHKGSDE SEGERRLTQS WPGPLACPQG PAPLASFQDD LDMDSSEGSS PNASPHDREE ACSPQQEPQG CRCPLDRFQD FALIDAPTLE DEPQEGQQWE IALPNNWQRY PRTKVEEKEA SDTGGEEPEK EEEDLYYGLP DGAGDLLPDK ELGFEPDTQG