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Recombinant human NHLH1 protein

The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. NHLH1 may serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system. Recombinant human NHLH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nescient helix loop helix 1, bHLHa35, HEN1, NSCL, NSCL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

17 kDa (156aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

NHLH1, Nescient helix loop helix 1, bHLHa35, HEN1, NSCL, NSCL1, ATGP2200-10 µg, ATGP2200-20 µg, ATGP2200-50 µg, ATGP2200-100 µg, ATGP2200-250 µg, ATGP2200-500 µg, ATGP2200-1 mg, ATGP2200-10, ATGP2200-20, ATGP2200-50, ATGP2200-100, ATGP2200-250, ATGP2200-500, ATGP2200-1

References & Citations

Brown L., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:8492-8496; ; Lipkowitz S., et al. (1992) J. Biol. Chem. 267:21065-21071;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005589

Uniprot Accession Number

Q02575

Species

Human

AA Sequence

MMLNSDT MELDLPPTHS ETESGFSDCG GGAGPDGAGP GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR VEAFNLAFAE LRKLLPTLPP DKKLSKIEIL RLAICYISYL NHVLDV

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