Recombinant human DPPA4 protein

DPPA4 may be involved in the maintenance of active epigenetic status of target genes and may inhibit differentiation of embryonic cells into a primitive ectoderm lineage. Recombinant human DPPA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Developmental pluripotency associated 4, 2410091M23Rik

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.9 kDa (327aa) confirmed by MALDI-TOF

Additionnal Information

DPPA4, Developmental pluripotency associated 4, 2410091M23Rik, ATGP2199-10 µg, ATGP2199-20 µg, ATGP2199-50 µg, ATGP2199-100 µg, ATGP2199-250 µg, ATGP2199-500 µg, ATGP2199-1 mg, ATGP2199-10, ATGP2199-20, ATGP2199-50, ATGP2199-100, ATGP2199-250, ATGP2199-500, ATGP2199-1

References & Citations

Ota T., et al. (2004) Nat. Genet. 36:40-45; ; Muzny D.M., et al. (2006) Nature. 440:1194-1198;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Development

NCBI Accession Number

NP_060659.3

Uniprot Accession Number

Q7L190

Species

Human

AA Sequence

MLRGSAS STSMEKAKGK EWTSTEKSRE EDQQASNQPN SIALPGTSAK RTKEKMSIKG SKVLCPKKKA EHTDNPRPQK KIPIPPLPSK LPPVNLIHRD ILRAWCQQLK LSSKGQKLDA YKRLCAFAYP NQKDFPSTAK EAKIRKSLQK KLKVEKGETS LQSSETHPPE VALPPVGEPP ALENSTALLE GVNTVVVTTS APEALLASWA RISARARTPE AVESPQEASG VRWCVVHGKS LPADTDGWVH LQFHAGQAWV PEKQEGRVSA LFLLPASNFP PPHLEDNMLC PKCVHRNKVL IKSLQWE