Recombinant human SPINK1 protein
SPINK1 is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. Recombinant human SPINK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Serine peptidase inhibitor Kazal type 1, Serine peptidase inhibitor, Kazal type 1, PCTT, PSTI, Spink3, TATI, TCP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
8.6 kDa (79aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Witt H., et al. (2000) Nat. Genet. 25:213-216; ; Hassan Z., et al. (2002) Am. J. Hum. Genet. 71:964-968;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_003113
Uniprot Accession Number
P00995
Species
Human
AA Sequence
DSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC
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