Recombinant human RCHY1 protein

RCHY1 has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Recombinant human RCHY1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

RING finger and CHY zinc finger domain-containing protein 1, ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.5 kDa (284aa)

Additionnal Information

RCHY1, RING finger and CHY zinc finger domain-containing protein 1, ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363, ATGP2187-10 µg, ATGP2187-20 µg, ATGP2187-50 µg, ATGP2187-100 µg, ATGP2187-250 µg, ATGP2187-500 µg, ATGP2187-1 mg, ATGP2187-10, ATGP2187-20, ATGP2187-50, ATGP2187-100, ATGP2187-250, ATGP2187-500, ATGP2187-1

References & Citations

Hu, L., et al. (2012) Carcinogenesis 33 (8), 1581-1588; ; Wang, J., et al. (2012) Pharmacogenomics J.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_056251

Uniprot Accession Number

Q96PM5

Species

Human

AA Sequence

MAATARE DGASGQERGQ RGCEHYDRGC LLKAPCCDKL YTCRLCHDNN EDHQLDRFKV KEVQCINCEK IQHAQQTCEE CSTLFGEYYC DICHLFDKDK KQYHCENCGI CRIGPKEDFF HCLKCNLCLA MNLQGRHKCI ENVSRQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HSALDMTRYW RQLDDEVAQT PMPSEYQNMT VDILCNDCNG RSTVQFHILG MKCKICESYN TAQAGGRRIS LDQQ