Recombinant human HLA-DOA protein

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. Recombinant human HLA-DAO protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

HLA class II histocompatibility antigen DO alpha chain, HLA class II histocompatibility antigen, DO alpha chain, HLA-DNA, HLA-DZA, HLADZ

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.1 kDa (215aa)

Additionnal Information

HLA-DOA, HLA class II histocompatibility antigen DO alpha chain, HLA class II histocompatibility antigen, DO alpha chain, HLA-DNA, HLA-DZA, HLADZ, ATGP2186-10 µg, ATGP2186-20 µg, ATGP2186-50 µg, ATGP2186-100 µg, ATGP2186-250 µg, ATGP2186-500 µg, ATGP2186-1 mg, ATGP2186-10, ATGP2186-20, ATGP2186-50, ATGP2186-100, ATGP2186-250, ATGP2186-500, ATGP2186-1

References & Citations

Yoon, T., et al. (2012) Proc. Natl. Acad. Sci. u.S.A. 109 (28), 11276-11281; ; Gieger, C., et al. (2012) Nature 480 (7376), 201-208

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002110

Uniprot Accession Number

P06340

Species

Human

AA Sequence

TKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME