Recombinant human PNRC2 protein

PNRC2 is involved in nonsense-mediated mRNA decay (NMD) by acting as a bridge between the mRNA decapping complex and the NMD machinery. This protein may act by targeting the NMD machinery to the P-body and recruiting the decapping machinery to aberrant mRNAs. It is required for uPF1/RENT1 localization to the P-body and also acts as a nuclear receptor coactivator. PNRC2 may play a role in controlling the energy balance between energy storage and energy expenditure. Recombinant human PNCR2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Proline-rich nuclear receptor coactivator 2, HSPC208

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18kDa (162aa)

Additionnal Information

PNRC2, Proline-rich nuclear receptor coactivator 2, HSPC208, ATGP2185-10 µg, ATGP2185-20 µg, ATGP2185-50 µg, ATGP2185-100 µg, ATGP2185-250 µg, ATGP2185-500 µg, ATGP2185-1 mg, ATGP2185-10, ATGP2185-20, ATGP2185-50, ATGP2185-100, ATGP2185-250, ATGP2185-500, ATGP2185-1

References & Citations

Hentschke, M., et al. (2003) Biochem. Biophys. Res. Commun. 312 (4), 975-982

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060231

Uniprot Accession Number

Q9NPJ4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGGGERY NIPAPQSRNV SKNQQQLNRQ KTKEQNSQMK IVHKKKERGH GYNSSAAAWQ AMQNGGKNKN FPNNQSWNSS LSGPRLLFKS QANQNYAGAK FSEPPSPSVL PKPPSHWVPV SFNPSDKEIM TFQLKTLLKV QV