Recombinant human Prohibitin 2 protein

PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases by similarity. This protein functions as an estrogen receptor (ER) -selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. PHB2 competes with NCOA1 for modulation of ER transcriptional activity. It is probably involved in regulating mitochondrial respiration activity and in aging. Recombinant human PHB2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Prohibitin 2, Bap37, p22, B cell receptor associated protein 37, BAP, BCAP37, PNAS-141, REA, PHB2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.7 kDa (322aa)

Additionnal Information

B cell receptor associated protein 37, BAP, BAP 37, BAP37, BCAP 37, BCAP37, p22, PHB2, PNAS 141, PNAS141, PNAS-141, Prohibitin 2, Prohibitin2, REA, ATGP2184-10 µg, ATGP2184-20 µg, ATGP2184-50 µg, ATGP2184-100 µg, ATGP2184-250 µg, ATGP2184-500 µg, ATGP2184-1 mg, ATGP2184-010, ATGP2184-020, ATGP2184-050, ATGP2184-100, ATGP2184-250, ATGP2184-500, ATGP2184-01M

References & Citations

Kategaya, L.S., et al. (2012) PLoS ONE 7 (2), E31987; ; Emerson, V., et al. (2010) J. Virol. 84 (3), 1355-1365

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001138303.1

Uniprot Accession Number

Q99623

Species

Human

AA Sequence

MAQNLKD LAGRLPAGPR GMGTALKLLL GAGAVAYGVR ESVFTVEGGH RAIFFNRIGG VQQDTILAEG LHFRIPWFQY PIIYDIRARP RKISSPTGSK DLQMVNISLR VLSRPNAQEL PSMYQRLGLD YEERVLPSIV NEVLKSVVAK FNASQLITQR AQVSLLIRRE LTERAKDFSL ILDDVAITEL SFSREYTAAV EAKQVAQQEA QRAQFLVEKA KQEQRQKIVQ AEGEAEAAKM LGEALSKNPG YIKLRKIRAA QNISKTIATS QNRIYLTADN LVLNLQDESF TRGSDSLIKG KK