Recombinant human GATC protein
GATC allows the formation of correctly charged Gln-tRNA (Gln) through the transamidation of misacylated Glu-tRNA (Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA (Gln) . This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glutamyl-tRNA (Gln) amidotransferase subunit C homolog, Glutamyl-tRNA (Gln) amidotransferase, subunit C homolog, 15E1.2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
17.5 kDa (159aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Nagao A., et al. (2009) Proc. Natl. Acad. Sci. u.S.A. 106:16209-16214
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_789788
Uniprot Accession Number
O43716
Species
Human
AA Sequence
MWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
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