Recombinant human PHF13 protein

PHD finger protein 13, also known as PHF13, is required for normal chromosome condensation during the early stages of mitosis and for normal chromosome separation during mitosis. Recombinant human PHF13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

PHD finger protein 13, PHF5, SPOC1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.0 kDa (323aa) confirmed by MALDI-TOF

Additionnal Information

PHF13, PHD finger protein 13, PHF5, SPOC1, ATGP2182-10 µg, ATGP2182-20 µg, ATGP2182-50 µg, ATGP2182-100 µg, ATGP2182-250 µg, ATGP2182-500 µg, ATGP2182-1 mg, ATGP2182-10, ATGP2182-20, ATGP2182-50, ATGP2182-100, ATGP2182-250, ATGP2182-500, ATGP2182-1

References & Citations

Kinkley S., et al. (2009) J. Cell Sci. 122:2946-2956; ; Ota T., et al. (2004) Nat. Genet. 36:40-45

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA, 250 mM Imidazole

Scientific Category

Cell Cycle

NCBI Accession Number

NP_722519

Uniprot Accession Number

Q86YI8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDSDSCA AAFHPEEYSP SCKRRRTVED FNKFCTFVLA YAGYIPYPKE ELPLRSSPSP ANSTAGTIDS DGWDAGFSDI ASSVPLPVSD RCFSHLQPTL LQRAKPSNFL LDRKKTDKLK KKKKRKRRDS DAPGKEGYRG GLLKLEAADP YVETPTSPTL QDIPQAPSDP CSGWDSDTPS SGSCATVSPD QVKEIKTEGK RTIVRQGKQV VFRDEDSTGN DEDIMVDSDD DSWDLVTCFC MKPFAGRPMI ECNECHTWIH LSCAKIRKSN VPEVFVCQKC RDSKFDIRRS NRSRTGSRKL FLD