Recombinant human C1GalT1/C1GalT1C1 protein

C1GALT1 generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from uDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis. Recombinant human C1GALT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Core 1 synthase glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, C1GALT, T-synthase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

41.4 kDa (357aa) confirmed by MALDI-TOF

Additionnal Information

C1GalT1, C1GalT1C1, Core 1 synthase glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, C1GALT, T-synthase, ATGP2181-10 µg, ATGP2181-20 µg, ATGP2181-50 µg, ATGP2181-100 µg, ATGP2181-250 µg, ATGP2181-500 µg, ATGP2181-1 mg, ATGP2181-10, ATGP2181-20, ATGP2181-50, ATGP2181-100, ATGP2181-250, ATGP2181-500, ATGP2181-1

References & Citations

Ju T., et al. (2002) J. Biol. Chem. 277:178-186; ; Zhu L., et al. (2009) Kidney Int. 76:190-198

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_064541

Uniprot Accession Number

Q9NS00

Species

Human

AA Sequence

LLGEKVD TQPNVLHNDP HARHSDDNGQ NHLEGQMNFN ADSSQHKDEN TDIAENLYQK VRILCWVMTG PQNLEKKAKH VKATWAQRCN KVLFMSSEEN KDFPAVGLKT KEGRDQLYWK TIKAFQYVHE HYLEDADWFL KADDDTYVIL DNLRWLLSKY DPEEPIYFGR RFKPYVKQGY MSGGAGYVLS KEALKRFVDA FKTDKCTHSS SIEDLALGRC MEIMNVEAGD SRDTIGKETF HPFVPEHHLI KGYLPRTFWY WNYNYYPPVE GPGCCSDLAV SFHYVDSTTM YELEYLVYHL RPYGYLYRYQ PTLPERILKE ISQANKNEDT KVKLGNP