Recombinant human NAT12/NAA30 protein

N-alpha-acetyltransferase 30, also known as NAA30, is catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. This protein catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. This protein is necessary for the lysosomal localization and function of ARL8B. Recombinant human NAA30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

N-alpha-acetyltransferase 30, C14orf35, MAK3, Mak3p, NAT12, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.7 kDa (385aa) confirmed by MALDI-TOF

Additionnal Information

NatC catalytic subunit, NAT12, NAT 12, N-alpha-acetyltransferase 30, N-acetyltransferase MAK3 homolog, N-acetyltransferase 12, NAA, N alpha acetyltransferase 30, N acetyltransferase MAK3 homolog, N acetyltransferase 12, Mak3p, MAK3, MAK 3, C14orf35, ATGP2180-10 µg, ATGP2180-20 µg, ATGP2180-50 µg, ATGP2180-100 µg, ATGP2180-250 µg, ATGP2180-500 µg, ATGP2180-1 mg, ATGP2180-010, ATGP2180-020, ATGP2180-050, ATGP2180-100, ATGP2180-250, ATGP2180-500, ATGP2180-01M

References & Citations

Starheim K.K., et al. (2009) Mol. Cell. Biol. 29:3569-3581

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001011713

Uniprot Accession Number

Q147X3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEVPPG PSSLLPPPAP PAPAAVEPRC PFPAGAALAC CSEDEEDDEE HEGGGSRSPA GGESATVAAK GHPCLRCPQP PQEQQQLNGL ISPELRHLRA AASLKSKVLS VAEVAATTAT PDGGPRATAT KGAGVHSGER PPHSLSSNAR TAVPSPVEAA AASDPAAARN GLAEGTEQEE EEEDEQVRLL SSSLTADCSL RSPSGREVEP GEDRTIRYVR YESELQMPDI MRLITKDLSE PYSIYTYRYF IHNWPQLCFL AMVGEECVGA IVCKLDMHKK MFRRGYIAML AVDSKYRRNG IGTNLVKKAI YAMVEGDCDE VVLETEITNK SALKLYENLG FVRDKRLFRY YLNGVDALRL KLWLR