Recombinant human Kallikrein 2/KLK2 protein

KLK2 is a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. KLK2 is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. Recombinant human KLK2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Kallikrein related peptidase 2, Glandular kallikrein-1, hGK-1, Tissue kallikrein-2, Prostatic kallikrein 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.5 kDa (260aa)

Additionnal Information

Kallikrein2, Kallikrein-related peptidase 2, hK2, KLK2A2, ATGP2175-10 µg, ATGP2175-20 µg, ATGP2175-50 µg, ATGP2175-100 µg, ATGP2175-250 µg, ATGP2175-500 µg, ATGP2175-1 mg, ATGP2175-10, ATGP2175-20, ATGP2175-50, ATGP2175-100, ATGP2175-250, ATGP2175-500, ATGP2175-1

References & Citations

Cargill M., et al. (1999) Nat. Genet. 22:231-238; ; Gan L., et al. (2000) Gene. 257:119-130

Other References

Lee SW, et al. Simple and robust antibody microarray-based immunoassay platform for sensitive and selective detection of PSA and hK2 toward accurate diagnosis of prostate cancer. (Sensing and Bio-Sensing Research, 2015) {https://doi.org/10.1016/j.sbsr.2015.01.003.}; ; Lee SW, et al. A Highly Sensitive Porous Silicon (P-Si) -Based Human Kallikrein 2 (hK2) Immunoassay Platform toward Accurate Diagnosis of Prostate Cancer. (Sensors (Basel) . 2015) {https://www.ncbi.nlm.nih.gov/pubmed/26007739}; ; Lee SW, et al. Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2. (Microchim Acta. 2016) {https://doi.org/10.1007/s00604-016-1986-1}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_005542

Uniprot Accession Number

P20151

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSIVGGWEC EKHSQPWQVA VYSHGWAHCG GVLVHPQWVL TAAHCLKKNS QVWLGRHNLF EPEDTGQRVP VSHSFPHPLY NMSLLKHQSL RPDEDSSHDL MLLRLSEPAK ITDVVKVLGL PTQEPALGTT CYASGWGSIE PEEFLRPRSL QCVSLHLLSN DMCARAYSEK VTEFMLCAGL WTGGKDTCGG DSGGPLVCNG VLQGITSWGP EPCALPEKPA VYTKVVHYRK WIKDTIAANP