Recombinant E.coli Maltose Binding Protein/MBP protein
Cytoplasmic maltose-binding protein, also known as MBP, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant E. coli MBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
44.9 kDa (410aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Fox JD., et al. (2001) Protein Sci. 10 (3) :622-30; ; Riggs P., et al. (2000) Mol Biotechnol. 15:51-63.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Fusion Tag & Detection
NCBI Accession Number
NP_418458.1
Uniprot Accession Number
P0AEX9
Species
E.coli
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items