Recombinant human TEF protein

Thyrotroph embryonic factor isoform 1, also known as TEF, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. TEF accumulates according to a robust circadian rhythm and has also been found to inhibit cell growth by down-regulating beta chain expression of cytokine receptors. Recombinant human TEF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thyrotroph embryonic factor isoform 1, KIAA1655, Thyrotroph embryonic factor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.6 kDa (326aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

TEF, Thyrotroph embryonic factor isoform 1, KIAA1655, Thyrotroph embryonic factor, ATGP2170-10 µg, ATGP2170-20 µg, ATGP2170-50 µg, ATGP2170-100 µg, ATGP2170-250 µg, ATGP2170-500 µg, ATGP2170-1 mg, ATGP2170-10, ATGP2170-20, ATGP2170-50, ATGP2170-100, ATGP2170-250, ATGP2170-500, ATGP2170-1

References & Citations

Khatib Z A., et al. (1994) Genomics. 23:344-351; ; Drolet D W., et al. (1991) Genes Dev. 5:1739-1753.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003207

Uniprot Accession Number

Q10587

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDAGGG KKPPVDPQAG PGPGPGRAAG ERGLSGSFPL VLKKLMENPP REARLDKEKG KEKLEEDEAA AASTMAVSAS LMPPIWDKTI PYDGESFHLE YMDLDEFLLE NGIPASPTHL AHNLLLPVAE LEGKESASSS TASPPSSSTA IFQPSETVSS TESSLEKERE TPSPIDPNCV EVDVNFNPDP ADLVLSSVPG GELFNPRKHK FAEEDLKPQP MIKKAKKVFV PDEQKDEKYW TRRKKNNVAA KRSRDARRLK ENQITIRAAF LEKENTALRT EVAELRKEVG KCKTIVSKYE TKYGPL