Recombinant human CNOT8 protein

CNOT8 belongs to the CAF1 family. It is a ubiquitous transcription factor required for a diverse set of processes and functions as part of the CCR-NOT complex. The CCR4-NOT complex functions as general transcription regulation complex. This complex contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8 and binds BTG2. Recombinant human CNOT8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CCR4-NOT transcription complex subunit 8, CAF1, CALIF, hCAF1, POP2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.9 kDa (315aa) confirmed by MALDI-TOF

Additionnal Information

CNOT8, CCR4-NOT transcription complex subunit 8, CAF1, CALIF, hCAF1, POP2, ATGP2161-10 µg, ATGP2161-20 µg, ATGP2161-50 µg, ATGP2161-100 µg, ATGP2161-250 µg, ATGP2161-500 µg, ATGP2161-1 mg, ATGP2161-10, ATGP2161-20, ATGP2161-50, ATGP2161-100, ATGP2161-250, ATGP2161-500, ATGP2161-1

References & Citations

Morel A.-P., et al. (2003) J. Cell Sci. 116:2929-2936; ; Albert T.K., et al. (2000) Nucleic Acids Res. 28:809-817

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004770

Uniprot Accession Number

Q9UFF9

Species

Human

AA Sequence

MPAALVE NSQVICEVWA SNLEEEMRKI REIVLSYSYI AMDTEFPGVV VRPIGEFRSS IDYQYQLLRC NVDLLKIIQL GLTFTNEKGE YPSGINTWQF NFKFNLTEDM YSQDSIDLLA NSGLQFQKHE EEGIDTLHFA ELLMTSGVVL CDNVKWLSFH SGYDFGYMVK LLTDSRLPEE EHEFFHILNL FFPSIYDVKY LMKSCKNLKG GLQEVADQLD LQRIGRQHQA GSDSLLTGMA FFRMKELFFE DSIDDAKYCG RLYGLGTGVA QKQNEDVDSA QEKMSILAII NNMQQ