Recombinant human HBG1 protein
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1) . The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5' - epsilon - gamma-G - gamma-A - delta - beta - 3'. Recombinant human HBG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Hemoglobin subunit gamma-1, HBGA, HBGR, HSGGL1, PRO2979
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
18 kDa (170aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Huisman TH, Kutlar F, et al. (1991) . Hemoglobin. 15 (5) :349-79.; ; Loudianos G, Moi P, et al. (1992) . Hemoglobin. 16 (6) :503-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_000550.2
Uniprot Accession Number
P69891
Species
Human
AA Sequence
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