Recombinant human HBG1 protein

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1) . The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5' - epsilon - gamma-G - gamma-A - delta - beta - 3'. Recombinant human HBG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hemoglobin subunit gamma-1, HBGA, HBGR, HSGGL1, PRO2979

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18 kDa (170aa) confirmed by MALDI-TOF

Additionnal Information

HBG1, Hemoglobin subunit gamma-1, HBGA, HBGR, HSGGL1, PRO2979, ATGP2153-10 µg, ATGP2153-20 µg, ATGP2153-50 µg, ATGP2153-100 µg, ATGP2153-250 µg, ATGP2153-500 µg, ATGP2153-1 mg, ATGP2153-10, ATGP2153-20, ATGP2153-50, ATGP2153-100, ATGP2153-250, ATGP2153-500, ATGP2153-1

References & Citations

Huisman TH, Kutlar F, et al. (1991) . Hemoglobin. 15 (5) :349-79.; ; Loudianos G, Moi P, et al. (1992) . Hemoglobin. 16 (6) :503-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000550.2

Uniprot Accession Number

P69891

Species

Human

AA Sequence

MGHFTEE DKATITSLWG KVNVEDAGGE TLGRLLVVYP WTQRFFDSFG NLSSASAIMG NPKVKAHGKK VLTSLGDATK HLDDLKGTFA QLSELHCDKL HVDPENFKLL GNVLVTVLAI HFGKEFTPEV QASWQKMVTA VASALSSRYH