Recombinant human SEMG1 protein
SEMG1 is the predominant protein in semen. The secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human SEMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Semenogelin-1, Semenogelin 1, CT103, dJ172H20.2, SEMG, SGI
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
52 kDa (462aa)
Additionnal Information
References & Citations
Brillard-Bourdet M, et al. (2002) . Eur J Biochem. 269 (1) :390-5
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.5M NaCl, 10% glycerol, 250 mM Imidazole, 0.1mM PMSF, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002998
Uniprot Accession Number
P04279
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
SLC14A1 (NM_001128588) Human Over-expression Lysate
