Recombinant human TOMM34 protein

TOMM34 is involved in the import of precursor proteins into mitochondria. The protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. Recombinant human TOMM34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitochondrial import receptor subunit TOM34, HTOM34P, TOM34, uRCC3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36.9 kDa (332aa) confirmed by MALDI-TOF

Additionnal Information

TOMM34, mitochondrial import receptor subunit TOM34, HTOM34P, TOM34, uRCC3, ATGP2149-10 µg, ATGP2149-20 µg, ATGP2149-50 µg, ATGP2149-100 µg, ATGP2149-250 µg, ATGP2149-500 µg, ATGP2149-1 mg, ATGP2149-10, ATGP2149-20, ATGP2149-50, ATGP2149-100, ATGP2149-250, ATGP2149-500, ATGP2149-1

References & Citations

Faou, P., et al. (2012) Biochim. Biophys. Acta 1823 (2), 348-357; ; Blesa, J.R., et al. (2008) Biochem. Cell Biol. 86 (1), 46-56

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006800

Uniprot Accession Number

Q15785

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPKFPD SVEELRAAGN ESFRNGQYAE ASALYGRALR VLQAQGSSDP EEESVLYSNR AACHLKDGNC RDCIKDCTSA LALVPFSIKP LLRRASAYEA LEKYPMAYVD YKTVLQIDDN VTSAVEGINR MTRALMDSLG PEWRLKLPSI PLVPVSAQKR WNSLPSENHK EMAKSKSKET TATKNRVPSA GDVEKARVLK EEGNELVKKG NHKKAIEKYS ESLLCSNLES ATYSNRALCY LVLKQYTEAV KDCTEALKLD GKNVKAFYRR AQAHKALKDY KSSFADISNL LQIEPRNGPA QKLRQEVKQN LH