Recombinant human Melan-A/MART-1 protein
Melanoma antigen recognized by T-cells 1, also known as MLANA, is involved in melanosome biogenesis by ensuring the stability of GPR143. This protein plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. Recombinant human MLANA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Melanoma antigen recognized by T-cells 1, MART-1, MART1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
10.4 kDa (94aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hoashi T., et al. (2005) J. Biol. Chem. 280:14006-14016; ; Giordano F., et al. (2009) Hum. Mol. Genet. 18:4530-4545
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_005502
Uniprot Accession Number
Q16655
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSCRRRNGY RALMDKSLHV GTQCALTRRC PQEGFDHRDS KVSLQEKNCE PVVPNAPPAY EKLSAEQSPP PYSP
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