Recombinant human FIBP protein

Acidic fibroblast growth factor intracellular-binding protein isoform a, also known as FIBP, is a member of Fibroblast growth factors (FGFs) represent a family. FGF activity influences development, adult tissue homeostasis, angiogenesis and cancer progression. FIBP is a 364 amino acid protein that binds to internalized FGF-1 and is thought to be involved in mitogenic function of FGF-1. FIBP localizes to the nucleus and is highly expressed in heart, skeletal muscle and pancreas and at lower levels in brain, placenta, liver and kidney. Recombinant human FIBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acidic fibroblast growth factor intracellular-binding protein, FGFIBP, FIBP-1, FIBP1, aFGF intracellular-binding protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.3 kDa (387aa) confirmed by MALDI-TOF

Additionnal Information

FIBP, Acidic fibroblast growth factor intracellular-binding protein, FGFIBP, FIBP-1, FIBP1, aFGF intracellular-binding protein, ATGP2145-10 µg, ATGP2145-20 µg, ATGP2145-50 µg, ATGP2145-100 µg, ATGP2145-250 µg, ATGP2145-500 µg, ATGP2145-1 mg, ATGP2145-10, ATGP2145-20, ATGP2145-50, ATGP2145-100, ATGP2145-250, ATGP2145-500, ATGP2145-1

References & Citations

Kolpakova E., et al. (1998) Biocham J. 336:213-222; ; Volkin D B., et al. (1996) Biotechnol. 9:181-217.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_942600

Uniprot Accession Number

O43427

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTSELDI FVGNTTLIDE DVYRLWLDGY SVTDAVALRV RSGILEQTGA TAAVLQSDTM DHYRTFHMLE RLLHAPPKLL HQLIFQIPPS RQALLIERYY AFDEAFVREV LGKKLSKGTK KDLDDISTKT GITLKSCRRQ FDNFKRVFKV VEEMRGSLVD NIQQHFLLSD RLARDYAAIV FFANNRFETG KKKLQYLSFG DFAFCAELMI QNWTLGAVGE APTDPDSQMD DMDMDLDKEF LQDLKELKVL VADKDLLDLH KSLVCTALRG KLGVFSEMEA NFKNLSRGLV NVAAKLTHNK DVRDLFVDLV EKFVEPCRSD HWPLSDVRFF LNQYSASVHS LDGFRHQALW DRYMGTLRGC LLRLYHD