Recombinant e.coli glpE protein
Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E. coli glpE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Thiosulfate:cyanide sulfurtransferase (rhodanese), ECK3411, JW3388
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.5 kDa (131aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Li H., et al. (2011) Biomol NMR Assign. 5 (1) :97-9; ; Ray WK., et al. (2000) J Bacteriol. 182 (8) :2277-84.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_417883
Uniprot Accession Number
P0A6V5
Species
E.coli
AA Sequence
MDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A
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