Recombinant human RNASEH2A protein

Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4) . Recombinant human RNASEH2A protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Specifications

Product Name Alternative

Ribonuclease H2 subunit A, AGS4, JuNB, RNASEHI, RNHIA, RNHL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.8 kDa (322aa)

Additionnal Information

RNASEH2A, Ribonuclease H2 subunit A, AGS4, JuNB, RNASEHI, RNHIA, RNHL, ATGP2138-10 µg, ATGP2138-20 µg, ATGP2138-50 µg, ATGP2138-100 µg, ATGP2138-250 µg, ATGP2138-500 µg, ATGP2138-1 mg, ATGP2138-10, ATGP2138-20, ATGP2138-50, ATGP2138-100, ATGP2138-250, ATGP2138-500, ATGP2138-1

References & Citations

Frank P., et al. (1998) Proc Natl Acad Sci uSA. 95:12872-12877.; ; Bayliss C D., et al. (2005) Nucleic Acads Res. 33:400-408.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006388

Uniprot Accession Number

O75792

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL