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Recombinant human IER3 protein

IER3 functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. Recombinant human IER3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Radiation-inducible immediate-early gene IEX-1, immediate early response 3, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

11.4 kDa (105aa) confirmed by MALDI-TOF

Additionnal Information

IER3, Radiation-inducible immediate-early gene IEX-1, immediate early response 3, DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1, ATGP2133-10 µg, ATGP2133-20 µg, ATGP2133-50 µg, ATGP2133-100 µg, ATGP2133-250 µg, ATGP2133-500 µg, ATGP2133-1 mg, ATGP2133-10, ATGP2133-20, ATGP2133-50, ATGP2133-100, ATGP2133-250, ATGP2133-500, ATGP2133-1

References & Citations

Kondratyev AD, Chung KN, Jung MO (May 1996) . Cancer Res 56 (7) : 1498-502; ; Wu MX, Ao Z, Prasad KV, Wu R, Schlossman SF (Aug 1998) . Science 281 (5379) : 998-1001

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Stem cell

NCBI Accession Number

NP_003888.2

Uniprot Accession Number

P46695

Species

Human

AA Sequence

MCHSRSC HPTMTILQAP TPAPSTIPGP RRGSGPEIFT FDPLPEPAAA PAGRPSASRG HRKRSRRVLY PRVVRRQLPV EEPNP

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