Recombinant human POU6F1 protein
POu6F1 belongs to the POu transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') . In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Recombinant human POu6F1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
POu domain class 6 transcription factor 1, POu domain, class 6, transcription factor 1, BRN5, MPOu, TCFB1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
35 kDa (324aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wey E, Lyons GE, et al. (1994) . Eur J Biochem. 220 (3) :753-62.; ; Messier H, Brickner H, et al. (1993) . Mol Cell Biol. 13 (9) :5450-60.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_002693
Uniprot Accession Number
Q14863
Species
Human
AA Sequence
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