Recombinant human ANKRD54 protein

ANKRD54, also known as LIAR, contains 4 ANK repeats. This protein plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. This protein interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN. It forms a multiprotein complex with LYN and HCLS1. ANKRD54 also interacts with TSN2, VAV1, DBNL AND LASP1. Recombinant human ANKRD54 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ankyrin repeat domain-containing protein 54, LIAR, Ankyrin repeat domain containing protein 54

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.9 kDa (323aa) confirmed by MALDI-TOF

Additionnal Information

ANKRD54, Ankyrin repeat domain-containing protein 54, LIAR, Ankyrin repeat domain containing protein 54, ATGP2122-10 µg, ATGP2122-20 µg, ATGP2122-50 µg, ATGP2122-100 µg, ATGP2122-250 µg, ATGP2122-500 µg, ATGP2122-1 mg, ATGP2122-10, ATGP2122-20, ATGP2122-50, ATGP2122-100, ATGP2122-250, ATGP2122-500, ATGP2122-1

References & Citations

Gustafsson, M.O., et al. (2012) Mol. Cell. Biol. 32 (13), 2440-2453 ; ; Olsen, J.V., et al. (2006) Cell 127 (3), 635-648

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_620152

Uniprot Accession Number

Q6NXT1

Species

Human

AA Sequence

MAAAAGD ADDEPRSGHS SSEGECAVAP EPLTDAEGLF SFADFGSALG GGGAGLSGRA SGGAQSPLRY LHVLWQQDAE PRDELRCKIP AGRLRRAARP HRRLGPTGKE VHALKRLRDS ANANDVETVQ QLLEDGADPC AADDKGRTAL HFASCNGNDQ IVQLLLDHGA DPNQRDGLGN TPLHLAACTN HVPVITTLLR GGARVDALDR AGRTPLHLAK SKLNILQEGH AQCLEAVRLE VKQIIHMLRE YLERLGQHEQ RERLDDLCTR LQMTSTKEQV DEVTDLLASF TSLSLQMQSM EKR