Recombinant human MED21 protein

MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mediator of RNA polymerase II transcription subunit 21, SRB7, SuRB7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

MED21, Mediator of RNA polymerase II transcription subunit 21, SRB7, SuRB7, ATGP2118-10 µg, ATGP2118-20 µg, ATGP2118-50 µg, ATGP2118-100 µg, ATGP2118-250 µg, ATGP2118-500 µg, ATGP2118-1 mg, ATGP2118-10, ATGP2118-20, ATGP2118-50, ATGP2118-100, ATGP2118-250, ATGP2118-500, ATGP2118-1

References & Citations

Baumli, S., et al. (2005) J. Biol. Chem. 280 (18), 18171-18178; ; Maldonado, E., et al. (1996) Nature 381 (6577), 86-89

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004255

Uniprot Accession Number

Q13503

Species

Human

AA Sequence

MADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS