Recombinant human MED21 protein
MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Mediator of RNA polymerase II transcription subunit 21, SRB7, SuRB7
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18 kDa (167aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Baumli, S., et al. (2005) J. Biol. Chem. 280 (18), 18171-18178; ; Maldonado, E., et al. (1996) Nature 381 (6577), 86-89
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004255
Uniprot Accession Number
Q13503
Species
Human
AA Sequence
Frequently Asked Questions
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