Recombinant human ACOT8 protein

Acyl-coenzyme A thioesterase 8, also known as ACOT8, are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. This protein may mediate Nef-induced down-regulation of CD4. It competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA) . ACOT8 shows a preference for medium-length fatty acyl-CoAs. Recombinant human ACOT8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.3 kDa (342aa) confirmed by MALDI-TOF

Additionnal Information

ACOT8, Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2, ATGP2115-10 µg, ATGP2115-20 µg, ATGP2115-50 µg, ATGP2115-100 µg, ATGP2115-250 µg, ATGP2115-500 µg, ATGP2115-1 mg, ATGP2115-10, ATGP2115-20, ATGP2115-50, ATGP2115-100, ATGP2115-250, ATGP2115-500, ATGP2115-1

References & Citations

Ishizuka M., et al. (2004) Exp. Cell Res. 297:127-141; ; Hunt M.C., et al. (2002) Prog. Lipid Res. 41:99-130

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_005460.2

Uniprot Accession Number

O14734

Species

Human

AA Sequence

MSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL