Recombinant human B3GAT3 protein
Beta-1, 3-glucuronyltransferase 3, also known as B3GAT3, is involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. This protein can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. It shows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Recombinant human B3GAT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Beta-13-glucuronyltransferase 3, Beta-1,3-glucuronyltransferase 3, GLCATI
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.4 kDa (330aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pedersen L.C., et al. (2000) J. Biol. Chem. 275:34580-34585; ; Tone Y., et al. (2008) J. Biol. Chem. 283:16801-16807
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_036332
Uniprot Accession Number
O94766
Species
Human
AA Sequence
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