Recombinant human B3GAT3 protein

Beta-1, 3-glucuronyltransferase 3, also known as B3GAT3, is involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. This protein can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. It shows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Recombinant human B3GAT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Beta-13-glucuronyltransferase 3, Beta-1,3-glucuronyltransferase 3, GLCATI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.4 kDa (330aa) confirmed by MALDI-TOF

Additionnal Information

B3GAT3, Beta-13-glucuronyltransferase 3, Beta-1,3-glucuronyltransferase 3, GLCATI, ATGP2114-10 µg, ATGP2114-20 µg, ATGP2114-50 µg, ATGP2114-100 µg, ATGP2114-250 µg, ATGP2114-500 µg, ATGP2114-1 mg, ATGP2114-10, ATGP2114-20, ATGP2114-50, ATGP2114-100, ATGP2114-250, ATGP2114-500, ATGP2114-1

References & Citations

Pedersen L.C., et al. (2000) J. Biol. Chem. 275:34580-34585; ; Tone Y., et al. (2008) J. Biol. Chem. 283:16801-16807

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_036332

Uniprot Accession Number

O94766

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQPCDCLP PLRAAAEQLR QKDLRISQLQ AELRRPPPAP AQPPEPEALP TIYVVTPTYA RLVQKAELVR LSQTLSLVPR LHWLLVEDAE GPTPLVSGLL AASGLLFTHL VVLTPKAQRL REGEPGWVHP RGVEQRNKAL DWLRGRGGAV GGEKDPPPPG TQGVVYFADD DNTYSRELFE EMRWTRGVSV WPVGLVGGLR FEGPQVQDGR VVGFHTAWEP SRPFPVDMAG FAVALPLLLD KPNAQFDSTA PRGHLESSLL SHLVDPKDLE PRAANCTRVL VWHTRTEKPK MKQEEQLQRQ GRGSDPAIEV