Recombinant human FBLIM1 protein

Filamin binding LIM protein 1, also known as FBLIM1, serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. This protein is implicated in cell shape modulation (spreading) and motility. It may participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. FBLIM1 promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. Recombinant human FBLIM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Filamin binding LIM protein 1, CAL, FBLP-1, FBLP1, RP11-169K16.5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

43.1 kDa (396aa) confirmed by MALDI-TOF

Additionnal Information

FBLIM1, Filamin binding LIM protein 1, CAL, FBLP-1, FBLP1, RP11-169K16.5, ATGP2113-10 µg, ATGP2113-20 µg, ATGP2113-50 µg, ATGP2113-100 µg, ATGP2113-250 µg, ATGP2113-500 µg, ATGP2113-1 mg, ATGP2113-10, ATGP2113-20, ATGP2113-50, ATGP2113-100, ATGP2113-250, ATGP2113-500, ATGP2113-1

References & Citations

Tu Y., et al. (2003) Cell. 113:37-47; ; Takafuta T., et al. (2003) J. Biol. Chem. 278:12175-12181

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_060026

Uniprot Accession Number

Q8WUP2

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASKPEK RVASSVFITL APPRRDVAVA EEVRQAVCEA RRGRPWEAPA PMKTPEAGLA GRPSPWTTPG RAAATVPAAP MQLFNGGCPP PPPVLDGEDV LPDLDLLPPP PPPPPVLLPS EEEAPAPMGA SLIADLEQLH LSPPPPPPQA PAEGPSVQPG PLRPMEEELP PPPAEPVEKG ASTDICAFCH KTVSPRELAV EAMKRQYHAQ CFTCRTCRRQ LAGQSFYQKD GRPLCEPCYQ DTLERCGKCG EVVRDHIIRA LGQAFHPSCF TCVTCARCIG DESFALGSQN EVYCLDDFYR KFAPVCSICE NPIIPRDGKD AFKIECMGRN FHENCYRCED CRILLSVEPT DQGCYPLNNH LFCKPCHVKR SAAGCC