Recombinant human Selenoprotein F (SC96C) protein

SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Recombinant human SEP15 (SC96C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

15 kDa selenoprotein, Selenoprotein F, SELENOF, SEP15

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.7 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

SEP15 (SC96C), 15 kDa selenoprotein, ATGP2106-10 µg, ATGP2106-20 µg, ATGP2106-50 µg, ATGP2106-100 µg, ATGP2106-250 µg, ATGP2106-500 µg, ATGP2106-1 mg, ATGP2106-10, ATGP2106-20, ATGP2106-50, ATGP2106-100, ATGP2106-250, ATGP2106-500, ATGP2106-1

References & Citations

Korotkov K.V., et al. (2001) J. Biol. Chem. 276:15330-15336; ; Kumaraswamy E., et al. (2000) J. Biol. Chem. 275:35540-35547

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004252

Uniprot Accession Number

O60613

Species

Human

AA Sequence

VSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI