Recombinant human CD74 protein
HLA class II histocompatibility antigen gamma chain isoform b, also known as CD74, is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. It is upregulated in several cancers and is also expressed by nonimmune cells during inflammation. Recombinant human CD74 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA, II
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
20.6 kDa (183aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wilson K M., et al. (1993) Immunology. 79:331-335; ; Henne C., et al. (1995) Immunology. 84:177-182.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_004346.1
Uniprot Accession Number
P04233
Species
Human
AA Sequence
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