Recombinant human RPL23A protein

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL23A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. Recombinant human RPL23A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosomal protein L23a, L23A, MDA20

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

20.1 kDa (179aa) confirmed by MALDI-TOF

Additionnal Information

RPL23A, Ribosomal protein L23a, L23A, MDA20, ATGP2102-10 µg, ATGP2102-20 µg, ATGP2102-50 µg, ATGP2102-100 µg, ATGP2102-250 µg, ATGP2102-500 µg, ATGP2102-1 mg, ATGP2102-10, ATGP2102-20, ATGP2102-50, ATGP2102-100, ATGP2102-250, ATGP2102-500, ATGP2102-1

References & Citations

Choudhary C., et al. (2009) Science. 325:834-840; ; Wang B., et al. (2008) Anal. Chem. 80:9526-9533

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 250 mM imidazole

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000975

Uniprot Accession Number

P62750

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAPKAKK EAPAPPKAEA KAKALKAKKA VLKGVHSHKK KKIRTSPTFR RPKTLRLRRQ PKYPRKSAPR RNKLDHYAII KFPLTTESAM KKIEDNNTLV FIVDVKANKH QIKQAVKKLY DIDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII