Recombinant human RPL23A protein
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL23A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. Recombinant human RPL23A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribosomal protein L23a, L23A, MDA20
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
20.1 kDa (179aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Choudhary C., et al. (2009) Science. 325:834-840; ; Wang B., et al. (2008) Anal. Chem. 80:9526-9533
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 250 mM imidazole
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_000975
Uniprot Accession Number
P62750
Species
Human
AA Sequence
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